Reliable EDC/NHS chemistry for confident, reproducible results
When it comes to covalent amine immobilization via EDC/NHS chemistry, the quality of your reagents makes all the difference. Impure or underperforming coupling reagents can silently undermine your assay – reducing binding capacity, compromising functionality, and eroding reproducibility. Unfortunately, these issues often surface only at the end of a workflow, when troubleshooting is most costly and time-consuming. That’s why we developed the Vixen Bio Amine Coupling Kit—a complete solution featuring SPR-graded EDC, NHS, and quenching reagents. With validated performance and lot-to-lot consistency, this kit ensures that your coupling strategy is both reliable and reproducible, whether you’re running routine assays or developing new methods.
Data to rely on
Many covalent coupling workflows happen “blind”, you only see results at the very end. Vixen Bio’s real-time detection method changes that by monitoring every step of the coupling process, providing full assay control. This makes it easy to spot issues early and demonstrates just how much reagent quality can affect your assay.
In a head-to-head comparability study, our Amine Coupling Kit showed:
- Improved inter-run reproducibility – CV% from 40.4% (unqualified) down to 3.2% (Amine coupling kit).
- 79.4% higher probe capacity (immobilization)
- 59.4% increase in binding efficiency (target)
These gains translate directly into more reliable data, stronger signals, and fewer failed experiments.
Why choose the Vixen Bio Amine Coupling Kit?
- Clarity, not troubleshooting – Stop guessing whether poor results are due to your coupling chemistry.
- Validated quality – Specifically tested to support surface plasmon resonance and other high-sensitivity applications.
- Consistent results – Reduce variability, avoid failed assays, and improve reproducibility
- Easy integration – Works seamlessly with Vixen Carboxyl Sensor Probes and is fully compatible with platforms like WHITE FOx, as well as other assays requiring certified activation and coupling.
Build confidence in your assays
Whether you’re an experienced SPR user or just starting with covalent coupling workflows, you can rely on the Vixen Bio Amine Coupling Kit to remove uncertainty from your experiments. Backed by real-time performance validation, our kit gives you the assurance that your coupling chemistry will work as expected -so you can focus on the science, not troubleshooting.
Ready to learn more?
Contact our experts at info@vixenbio.com or reach us through our contact form to
discuss how the Amine Coupling Kit can support your research.